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ѧϵ

־㲥ӵý嶼л򹤳̼ĽܡѧѾ˽һЩ򹤳̵ݣ֪˵ػбʹѸ٣֪ˮĿֲбӶܸߵֲڻ򹤳̵ĵʵ΢ֲ֮Ļǰʵֵ˼뽫ΪʵʵУתҲǵߣаڷתեֲͣתĿޣҩƷ̵ת΢ȵء˵ݶѧѧϰ֪ʶϵľ顣

һЩ޿ѧϰ֪ʶΪѧϰ֪ʶ̵档

9

ѧ QQȺ109891693 ӭϹѧ

ѧϰøDNAøʱޱйDNAṹ֪ʶϵDNAṹĻ֪ʶܽϺõøĹܡ

ѧϰĿĻʱޱDNAָʺϳɵĹϵѧ⣬ΪʲôҪϼ⣺ټתǷĿĻ򣻢ڼĿĻǷת¼mRNAۼĿĻǷɵʡ

ѧϰĿʱϵޱƻݣӶһЩʶѧϰcDNAĿʱϵDNAת¼й֪ʶmRNAΪģ壬ת¼ɻһDNAԵΪģϳ˫DNA

ѧϰPCRʱϵޱеDNAƵݡ

ѧϰ򹤳̵ӦһʱӦѧϵеľѧ˼û򹤳̵ķʵ⡣

ġ רϵ

רݶרнܵϵתѧϰ֪ʶĻӦѧѧϰ\İȫԺ⡷ר⡣̡̥йֲ̥ݣʹѧתи͸⡣ϸ̡нֲϸĿĻֲϸתֲҪڡ⣬ѧϰרʱйע̡̬רгֵ̬⣬˼û򹤳̵ķ̬г漼Խ⡣ 1.1 DNA鼼Ļ

һ ѧĿ

1.DNA鼼ֻߵá

2.ͬ򹤳̵ĵͷչ벻оͼ¡ ѧصѵ 1.ѧص

DNA鼼ֻߵá 2.ѧѵ

򹤳Ҫ߱ ѧ

1.龳ѧ˼ѧϰ֪ʶ

ҪǽDNA鼼ֻ߼áDzֱסƽķʽܣڵѧѧϰĻԣҲѧѧȫߡӦͨ龳⣬յѧѧ˼բš

øҪǽøãиĽڽⲿѧϰʱѧĵ⡰øѰҡյѧǰѧݩɾȾϸʵ飬ʶϸȵϸܵȻԴDNA֡ôԭ֮ԳڽкαƣյѧʲôøиԴDNAʹ֮ʧЧﵽĿġͽֱ׵ġøҪǴԭз봿дһ̽˼

DNAøDNAƬεġӷ롱дñȽϼࡣǿԴԭе֪ʶշѧ˼ﵽáҪӱиDNAѧݴǰѧ֪ʶһӦ뵽DNAۺøѧ뷨IJǺȻġʵϲøDNAƬεӡӦѧDNAۺøDNAøIJͬãӶﵽʶDNAøĿġ

ϸ婤䳵ѧϰݣᵽΪĸѧܽѧס⼸Ӧͨյ˼ȷΪʲôҪĸܳ䵱塣

2.óֱ۵IJͼҵעͣʵʶγȷʶ

10

ѧ QQȺ109891693 ӭϹѧ

־г󡢸ص㣻ͼϢý壬ֱ۵ص㣬ױ֪⣬󡢸ܲҪⱾеĺ壬ʦ뽫IJͼȷϢýлؽƻѧһʱĥ͸ijԣھ塢ֱ۵IJͼҵע⡣

ĩˡƽĩ˵ԼIJλֱ۵IJͼЭͬãʹѧ׼ȷиӲλ⡰ԡںDNAøǡϡģDNAеںδϲͼ⡣ܽṹģʽͼѧҲʹѧԹйݱ⡣

ģDNAģʱֹֻּֽͶģǸѧںĶֹ̡ʱʶһEcoRIӦȥѰG AATTCļУȻGA֮ɽʱʶֻȥԴģʧʱҲҪ룬ʵ¿ʲôԭ¡

3.ѧӻ򹤳̵˼⣬ڽѧѵ㡣

ڵѵǶ߱ķҪ⣬µЧֻнֲϵ򹤳̵ƹв⡣磬ѡôӻһе壬ϻ򹤳̵ʵĿ룺˭ϸ壿ȻǶԷĻúйǣӶϸ޺ȻҪ岻кϸӰС

磬ûбǻֿǷ룬ôμֻ뵽ʵʹⷽѣŻԤΪʲôҪѡ߱ǻ塣

磬ûһͶиλ㣬ͲܽDNA顣DNAܸƣͿܶʧԽʦҪѧһпǣֲΪʵĿꡣ

ġʾ һ˼̽

1.øDNAκβλܽDNAпֲͬøиγɵDNAƬΣ (1) CTGCA (2) AC (3) GC G TG CG 4 G (5) G (6) GC CTTAA ACGTC CG (7) GT (8)AATTC CA G

ǷDNAø

27γɡACGT TGCA

48γɡGAATTC CTTAAG 36γɡGCGC CGCG

15γɡCTGCAG GACGTC

2.ϵе֪ʶһ룬ΪʲôϸøϸDNA

ʾΪֹ򹤳ʹõø󲿷ֶǴϸùȡģǸԿʶжDNAضļСϸø֮ԲжDNAΪ΢ڳڵĽγһƵķƣԴֵDNAԽڳݻУijøϸ

11

ѧ QQȺ109891693 ӭϹѧ

DNAл߲߱øʶиУͨ׻ø׻תƵʶеļϣʹøܽпϸкijøҲʹDNAжϣҿԷֹԴDNA֣ⲻҪѧشȫʦɲοʦеʾѧľָ

ԭҲӦ½йĻش𡣣

3.ȻDNAӿֱ򹤳Ϊʲô

ʾ򹤳ΪʹõDNAӺܶ඼plasmidϸ˴ȾɫDNA֮һֿҸơ˫ջ¶DNAӡǷκΪ򹤳ʹأʵȻΪ򹤳ʹõ

1 DNAһøиλ㣬ԱĿĻԲ뵽ȥЩĿĻøеλãҪĻƬ֮⣬ŲĿĻIJʧ

2 DNA߱ҸƵϵȾɫDNAȾɫDNAĸƶͬơ

3 DNAбǻԱӵɸѡ

4 DNAǰȫģϸкܽ뵽ϸϸȥ 5 DNAӴСӦʺϣԱȡв̫Ͳ

ʵȻڵDNAӲȫ߱Ҫ˹ڻ򹤳̲ 4.ϲѯDNAøӵDNAı

ʾΪֵֹDNAøӵDNAԭڻҲֿᷢӵDNAø

Ѱʵ

1.յ֪ʶܷøԭеʲô

ʾԭܵȻԴDNA֣ǣڳڵĽγһƵķƣԷֹԭֺøϸһַԹߣԴDNAʱøԴDNAиԱ֤İȫԣøԭҪиԴDNAʹ֮ʧЧӶﵽĿġ

2. DNAøDNAۺøһΪʲô

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