南通职业大学毕业设计（论文） 1-氨基蒽醌的测定

摘 要

采用高效液相色谱法，用甲醇与水的混合溶液作为流动相，调节流动相的比例和流

速，根据出峰情况及相应保留时间的情况，从而得到测量的最佳条件，甲醇：水=85:15，用校正峰面积归一化法测定1-氨基蒽醌的含量，通过对重复性、线性、相对偏差及回收率的测定，对该测量方法进行验证，通过测量，该方法的重复性和线性都较好，精密度和回收率也较高。从而可以判定该方法简便、准确，是测定1-氨基蒽醌含量的一种好方法。

关键词：1-氨基蒽醌，高效液相色谱法，方法验证

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南通职业大学毕业设计（论文） 1-氨基蒽醌的测定

Abstract

Using high performance liquid chromatography, using methanol and water mixed solution as mobile phase, regulating the proportion of mobile phase and flow rate, according to a peak and the corresponding retention time, and thereby obtain the best conditions for measurements, methanol: water = 85 : 15, with the corrected peak area normalization method determination of 1 - amino-anthraquinone content, through the repeatability, linearity, relative deviation and recovery such as the determination of the measurement method to verify, by measuring, the method of have good repeatability and linearity, precision and recovery is also high. And thus can determine the method is simple, accurate, is to determine the 1 - amino-anthraquinone content in a good way.

Keywords: 1 - amino-anthraquinone, high-performance liquid chromatography, method

validation

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南通职业大学毕业设计（论文） 1-氨基蒽醌的测定

目 录

摘 要……………………………………………………………………1 Abstract…………………………………………………………………..2 1 引言…………………………………………………………………….5

1.1理化性质…………………………………………………………………………….......5 1.1.1物理性质……………………………………………………………………5 1.1.2化学性质……………………………………………………………………5 1.21-氨基蒽醌的生物活性…………………………………………………………………5 1.2.1毒性………………………………………………………………………….5

1.2.2刺激性……………………………………………………………………….6 1.2.3反应活性…………………………………………………………………….6 1.3接触控制与个体防护…………………………………………………………………..6 1.3.1接触控制……………………………………………………………………6 1.3.2防护…………………………………………………………………………6 1.4生产工艺…………………………………………………………………………………6 1.4.1氨化氨解法…………………………………………………………………………6 1.4.2硝化还原法…………………………………………………………………………7 1.4.3催化加氢法…………………………………………………………………………7 1.4.4磺化氨化法………………………………………………………………………….7 1.5废弃处置与运输…………………………………………………………………………8 1.5.1废弃处置…………………………………………………………………….8 1.5.2运输………………………………………………………………………….8 1.6泄露应急措施…………………………………………………………………………….9 1.6.1应急处理……………………………………………………………………..9

1.6.2小量泄漏……………………………………………………………………..9 1.6.3大量泄漏……………………………………………………………………..9 1.7操作与储存………………………………………………………………………………..9

1.7.1操作………………………………………………………………………….9 1.7.2储存………………………………………………………………………….9 1.8应用………………………………………………………………………………………..9

2 1-氨基蒽醌的测定………………………………………………………10

2.1高效液相色谱法…………………………………………………………………………10 2.2薄层色谱法………………………………………………………………………………10

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南通职业大学毕业设计（论文） 1-氨基蒽醌的测定

2.3方法比较…………………………………………………………………………………10

3 实验部分…………………………………………………………………….11

3.1高效液相色谱法测定1-氨基蒽醌……………………………………………………11

3.1.1方法提要…………………………………………………………………………11 3.1.2试样和溶液………………………………………………………………………11 3.1.3仪器……………………………………………………………………………….11 3.1.4色谱分析条件……………………………………………………………………11 3.1.5标准溶液的制备及校正因子的测定……………………………………………12 3.1.6分析步骤…………………………………………………………………………12 3.1.7允许差……………………………………………………………………………13 3.1.8测定和计算………………………………………………………………………13 3.1.9分析方法的验证…………………………………………………………………14 3.2结果分析………………………………………………………………………………16

参考文献……………………………………………………………………….17 致 谢…………………………………………………………………………18

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