42青鳞鱼鳃内Na。K+-ATPase基因的克隆及皮质醇对其表达的影响AmericanZoologist,2000,40(3):393-401.【18]支0克嘉,邬勤娥.应激与应激性疾病【M】.人民军医出版社,1991.【19]1{1家基本药物领导小组.国家基本药物临床手册【M】.华夏出版社,1999.【20]林浩然,鱼类生理学[M1.广东高等教育出版社,1999,【21]TripathiGVermaP.Sex—specificmetabolicchangesintheannualreproductivecycleofafreshwatercatfish[J].Comp.Biochem.Phys.B.,2004,137(1):101—106.【22]MarshallWS.Rapidshort-durationfreshwaterregulationofNaCIsecretionbyestuarineteleostfish:copingstrategiesforexposures[J].Bba-Biomembranes,2003,1618(2):95-105【23]Fontainhas-FemandesosmoregulatoryA,GomesEF,Reis-HenriquesMA,eta1.Effectofcortisolonsomeparametersoftheteleostaftertransferencefromfreshwamrtoseawater[J].Arq.Bras,Med.Vet.Zoo.,2003,55(5):562-567.【24]DeaninaDB,WhitlowZw'BomkiRJ.Glucocorticoidreceptorupregulationduringseawateradaptationeuryhalineteleost,thetilapia(OreochromisMossambicus)【J】.Gen.Comp.Endocr.,2003,132(1):112—118.125]LeonardiMrainbow0,KlempauAE.Artificialphotoperiodinfluenceontheimmunesystemofjuveniletrout(OncorhynchusMy船s)intheSouthernHemisphere[J].Aquaculture,2003,221(1-4):581-591.on【26]McCormickMinal,1',lechaevlV.Influenceofcortisnldevelopmentalrhythmsduringembryogenesistropicaldamselfish[J].J.Exp.Z001.,2002,293(5):456-466.N【271Pankhurstrainboww,VaninDerKraak,GEvidenceThatacutestressinhibitsovariantheactionofsteroidoganesisandintroutvivo,throughcortisol[J].GeneralComparativeEndocrinology,2000,117(2):225—237.【28]T.韧.医药及其中间体的发展趋鲫【J】.化工技术经济,2003,21(7):1—10.【29]http://www.sw2000.com.cn/sw2000/zx/swsd/xgxs/userobjecttai559047.htrnl【30]JorgensentheArcticEH,ByeBE,JoblingM.InfluenceofnutritionalstatusonbiomarkerresponsestoPCBinchaff(SalvelinusAlpinus)【J】.AquaticToxicology,1999,44(4):233—244.P【31】ColinWafingA,AndrewMooreb.TheeffectofatrazineseaonAtlanticsalmon(Salmosalar)smoltsinfreshwaterandafterwatertransfer[J],AquaticToxicology,2004.66(1):93-104.of【32]HandyRD.Chroniceffectscopperexposureversusendocrinetoxicity:TwosidesofthesamePhysiologytoxicologicalprocess?【J】.ComparativeBiochemistryandA?Molecular&IntegrativePhysiology,2003,135(1):25—38,【33]MontgomeryRA,DallmanMJ.Semi—quantitativepolymerasechainreactionanalysisofcytokineandcytokinereceptorgeneexpressionduringthymicontogeny【j】.Cytokine,1997,97:17-26.transcription-polymerasechainreaction【34]WallSJ,EdwardDR.QuantitativereversefrR-PCR):Acomparisonofprime-dropping,competitive,andreal—timeRT-PCRsIJ】-AnalBiochemistry,2002,300(2):269—273.【35]WillardMF,Stephen1999,26(1):112-125.【361GiuliettiA,OverberghL,ValckxD,eta1.Anoverviewofreal-timequantitativePCR:applicationtoJW,KentEV.QuantitativeRT—PCR:PitfallandPotential【J】.BioTechniques,quantifycytokinegeneexpression【J】.Methods,2001,25(4):386—401.PCR【J1.GenomeRes,【37]Held,CA,StevensJ,LivakJKeta1.Realtimequantitative参考文献431996,6:986-994.【38]WittwerDNACT,HerrmannMG,MossAA,eta1.Continuousfluorescencemonitoringofrapidcycleamplification[J】.Biotechniques,1997,22:130-138.TH,Pate/Bl(’CincoM,eta1.IdentificationofLeptospirabiflexabyreal-timehomogeneous【39]WoodetectionofrapidcyclePCRproduct【J】.JournalofMicrobiologicalMethods,1999,35:23-30.reverse【40lYinchainJL,ShackelNA,ZekryA,McGuninnessPH,eta1.Real—timeofcytokineandtranscriptase-lx)lymerasemRNAexpressionwithreaction(RT-PCR)formeasurementorgrowthfactorflourogenicprobeSYBRGreenI【J】.ImmunologyandCellBiology,2001,79:213—221【41]McCormickSD.EndocrinecontrolofosmotegulationinteleosttjshtJl.flu'neronZool,2001,41:781-794,【42]PelisRM,McCormickSD.EffectsofgrowthhormoneandcortisolNa+,K+,一CI—cotransporterlocalizationandabundanceinthegillsofAtlanticsalmon[J].GenCompEndocrinol,2001.124:134--143.【43]W.S.Marshall,S.E.Bryson.Transportmodelofamechanismsofseawaterteleostchloridecells:aninclusivemultifonctionalceu【J】.Comp.Biochem.Physi01.A,1998,119:97-106.a1.GeneexpressionofNa+一K+-ATPasealanda3subunitsingillsto[441s.H.Feng,J.H.Leu,C.H.Yang,etoftheteleost(Oreochromismossambicus)adapteddifferentenvironmentalsaimities,Mar[J].Bioteclmol,2002.4:379_-39l,【45]J.W.Semple,HJ.Green,P.M.Schulte.MolecularisoformsinFunduluscloningandcharacterizationoftwoNa/K-ATPaseheteroclitus[J].Mar.Biotechn01.2002.4:512-519.alpha【461J.G,Ricbards,J.W.Semple,J.S,Bystriansky,eta1.Na(+),l((+)-ATPasegillsofrainbowisoformswitchingintrout(Oncorhynchusmykiss)duringsalinitytransfer[J],J.Exp.Bi01.2003.206:4475-4486.andbeta[47]B.Blasiole,A.Degrave,V.Can.eld,eta1.DifferentialexpressionofNa,K-ATPasealphasubunitgenesinthedevelopingzebrafishinnerear[J].Dev.Dyn.,2003,228:386-392.cloningandcharacterizationof【48]J.W.Semple,HJ.Green,P.M.Schulte,MolecularisoformsinFundulustwoNa/K.ATPase‘heteroclitus,Mar.Bioteclmol,4(2002)512-519.Yan,】.H.Postlethwait,K.C.Cheng,R.Levenson,149]SJ.Rajarao,V.A.Can.eld,M.A.Mohideen,Y.LTherepertoireofNa,KATPasealphaandbetasubunitgenesexpressedinthezebrafishDaniorerio,GenomeRes.,11(2001)1211—1220.Geering,R,A,Farley,Thesodiumpumpneedsits[50]A.A.McDonough,k1990,4:1598-1605.b-subunit[J].FASEBJ.,[511温海深,高玲,李文阁.硬骨鱼类神经内分泌,免疫网络及其调控研究_中国海洋大学学报,2006,36(1):60~64【52]MareY,EngelsmaMO,HuisingWB,eta1.Neuroendocrineimmuneinteractionsinfish:aroleforinterlenkin21【Jj,Vet【53]Ainsworthlmmun01.,2002,87:467-479.AJ,DexiangC,WaterstratPR.ChangesinperipheralbloodleukocytepercentagesandAquatAnimHealth,1991,3:41-47.onfunctionofneutrophilsinstressedchannelcatfish[J】.J【54]EspelidS,LokkenGB,SteiroKeta1.EffectsofcortisolandstresstheimmunesysteminAtlanticSalmon(SalmosalarL.)【J1.Fish[55]AltschulSF,MaddenShellfishImmun01.,1996,6:95.110BLASTandPSIBLAST:AuewTL,SchafferAA,ZhangJ,eta1.Gappedgenerationofproteindatabasesearchprograms[J].NucleicAcidsResearch,1997,25:3389—3402【56】沈同,王镜岩.生物化学(下册).高等教育出版社[MI,1991,30.32[57]C.P.Cutler,I.L.Sanders,N.Hazon,ela1.Primarysequence,tissuespecificityandmRNAexpressionof竺the童塑鱼塑堕竖:兰二!!翌竺堇望塑塞堕垄堡堕壁翌茎室垄塑星堕Na++F-ATPaseB1subunitintheEuropeaneel(Anguillaanguilla)【J】FishPhysi01.Biochem,1995,14:423--429[58】sJ.Rajarao,VACaneld,M.A.Mohideen,eta1.nerepertoireofNa+.K+.ATPasealphaandbetasnbunitgenesexpressedinthezebrafish(Daniorerio)fJ】,GenomeRes.,2001,11:1211—1220expressionofNa,K-ATPasealphaandbeta【591B.Blasiole,A.Degrave,V.Can.eld,eta1.Differentialsubunitgenesinthedevelopingzebrafishinnereat【J】,Dev.Dyn.,2003,228:386-392【60]MauleA…GSchreckC…BStressandcortisoltreatmentchangedaffinityandnumberofglucocorticoidreceptorsinleukocytesandginofcohosalmon【J】.Gen.Comp.Endocrinol,1991,84:83-93.et(61]PriceS.R.,EnglandB.K,BaileyJ—La1.AcidosisandglucocorticoidsconcomitantlyincreaseubiquitinandproteasomesubunitmRNAsinratmuscle【J】.Am.J.Physi01.Cell.Physi01.1994,267,C9552960.【62]DucoretB.,TujagueM.,AshrafJ.,eta1.Cloningcontainsaofteleostfishglucocorticoidreceptorshowsthatitdeoxyribonucleicacid-bindingdomaindifferentfromthatofmammals[J].Endocrinology,1995,136,3774-3783,[63]MathilakathM.Vijayan,StavroulaglucocorticoidreceptorComparativeRaptis,RameshSathiyaa.Cortisoltreatmentaffectsandglucocorticoid-responsivegenesintheliverofrainbowtroutfJl.GeneralandEndocrinology,2003,132:256-263【64】阳成波,印遇龙,龚建华等.实时定量PeR研究进展及其应用[J】.中国预防兽医学报,20035(25):395—399[65]MadsenS.S..TheroleofcortisolandgrowthhormoneinseawateradaptationanddevelopmentofseahypoosmoregulatorymechanismsinEndocrin01.,1990b,79:1-11trout(parrSalmotruttatrutta)[JJGen.Comp.【66]JensenM.K.,MadsenS.S,,KristiansenK。OsmoregulationandsalinityeffectsseaontheexpressionandactivityofNa,K-ATPaseinthegillsofEuropean1998.282:29m.300bass(Dicentrarchuslabrax)[J].J.Exp.Z001.,(67]KellyS.P.,ChowI.N.k,WooN.Y…SHaloplasticityofblackseabream(Mfliomacrocephalusj:hypersalinetofreshwateracclimation【J】.J.Exp.Z001.,1999,283:226-241a1.Cortisolreceptorblockadeandseawateradaptationintheofexperimentalzoology,2005,303A:132—142【68]W.SMarshall,R.R.F.Cozzi,R.M.PelisetEuryhalinetelost(Fundulusheteroclitus)【J】.Journal【69]BuryteleostNR,SturmA,LeRouzicP,ela1.Evidencefortwodistinctfunctionalglucocorlicoidreceptorsinfish[J].JMolecEndocrinol,2003.31:141—156NR,ela1.Amineralocorticoid-likereceptorintherainbowtrout.[70]ColombeLFostierA,BuryOncorhynchusmykiss:CloningandcharacterizationofitssteroidbindingSteroids,2000.65:319—328domain[J].[71]CrockettEL.LipidrestructuringdoesnotcontributetoelevatedactivitiesofNa+/K+-ATPaseinbasolateralmembranesfromthegillofseawater‘acclimatedBi01.,1999,202:2385—2392eel(Anguillarostrata)【11.JExp【721DeaneEE,Woosarba)【J】.AmNYS—DifferentialgeneexpressionassociatedwitheuryhalinityinseabreamfSparusJPhysi01.,2004,287:R1054-R1063Butler[73]GreenwoodAKPC,WhiteRB,eta1.Multiplecorticoidreceptorsinateleostfish:Distinctsequences,expressionpatterns,andtranscriptionalactivities[J].Endocrinology,2003,144:4226-4236茎耋苎壁竺【74]HansenHJM,GrosellM..Aremembranelipidsinvolvedinosmoregulation?Studiesinvivoontheeuropeaneel,Anguillaanguilla,afterreducedambientsalinity[J].EnvBiolFishes,2004,70:57--65【75]SingerTD,ClementsKM,SempleJW,eta1.SeawatertoleranceandgeneexpressionintwostrainsofAtlanticsalmonsmolts[J].CanJFishAquatSci,2002,59:125—135【76]SingerTD,FinstadB,McCormickSD,eta1.InteractiveeffectsofcortisoltreatmentandambientseawaterchallengeongillNa+/K+-ATPaseandCFTRexpressionintwostrainsofAtlanticsalmonsmolts[J].Aquaculture,2003,222:15~28【77]SlomanKA,DesforgesPR,GilmourKM..Evidenceformineralocorticoid.1ikereceptorlinkedtobranchialchloridecellproliferationinfreshwaterrainbowtrout[J].JExpBiol,2001,204:3953-3961.【781KojiInoue,YoshioTakei.ReviewAsianmedakafishesoffernewmodelsforstudyingmechanismsofseawateradaptation[J1.ComparativeBiochemistryandPhysiologyPartB,2003,136:635“45【79]http://mbase.bioweb.ne.jp/【80]http://www.tigr.org/tdby/tgi/olgi/.【81]http://bioll.bio.nagoya-U.ac.jp:8000/ISMG’03C.html【82]MauleA.G.,SchreckC.B.,Glucocorticoidreceptorsinleukocytesandgillofiuvenilecohosalmon(Oncorynchuskisutch)[J1.Gen.Comp.Endocfinol,1990,77:44&-455[8310’MalleyB.W.,TsaiM。Molecularpathwaysofsteroidreceptoraction[J1.Bi01.Reprod,1992,46:163-167【84]PelisR.M.,ZydlewskiJ.,McCormick,S.D。GillNa+一K+-2CI.cotransporterabundanceandlocationinAtlanticsalmon:Effectsofseawaterandsmolting[J].Am.J.Physi01.,2001,280:R1844-R1852.’。[85]McCormickS.D。HormonalcontrolofgUlNa+.一-ATPaseandchloridecellfunction[J]FishPhysiology,1995,14:285-315【86]DangeA.D..BranchialNa+一K+.ATPaseactivityinfreshwaterorsaltwateracclimaledtilapia(Oreochrom如Sarotherodon)mossambicus:effectsofcortisolandthyroxine[J].Gen.Comp.Endocrin01.,1996,62:341-343【871LinL.,WengC.,HwangP—Effectsofcortisolandsalinitychallengeonwaterbalanceindevelopinglarvaeoftilapia(Oreochromismossambicus)【J1.Physinl.Biochem.Z001.,2000,73:283-289.【88]McCormick,s.D..Conisoldirectlystimulatesthedifferentiationofchloridecellintilapiaopercularmembrane[J].Am.J.Physi01.,1990,259:R857-R863.【89]MadsenS.S,,JensenM.k,KristiansenK,eta1.ExpressionofNa+-K+-ATPaseinthebrowntrout,Salmotrutta:invivomodulationbyhormonesandseawater【J】.Am.J.Physi01.,1995,269:R13339-R1345[90]SunnyF.,Oommen0.V~RapidactionofglucocorticiodsonbranchialATPaseactivityin(Oreochromismossambicus):aninvivoandinvitrostudy.Comp.Biochem.Physi01.,2001,B130:323-330